鸡内源白血病病毒ev21与慢羽非连锁分析和LTR区启动子活性分析

doi:10.11843/j.issn.0366-6964.2017.05.018

Abstract

Abstract:

The research was designed to analyze the linkage between the endogenous avian leucosis virus ev21 and phenotype of late feathering, as well as the promoter activity of LTR of ev21 gene. It was aimed to find the detection method for ev21 free breeding chickens. Long-range PCR was used to amplify the sequence of ev21 gene. The structure and its locus in chicken chromosome were analyzed based on the NCBI database. Two hundred and fifty-nine samples from 5 chicken lines were diagnosed for the detection of ev21. And 5' and 3' LTR were cloned into the pGL3- basic vector and were transiently transfected into A375 cells. The relative luciferase activity, which indicted the promoter activity of the transfected fragments, was detected. It was showed that the genome of ev21 long for 7 524 bp was successfully cloned and it was integrated between g.10681671-10681672 on chicken Z chromosome in reverse direction. Ev21 and late feathering were completely linked in Hy-line Gray, but were incompletely-linked in Taihang, Bashang and Hy-line Brown chicken. Especially, it was all positive in Hy-line Brown fast-feather rooster. 5' LTR of ev21 showed a high promoter activity (P<0.01), while 3' LTR did not show significantly higher activity than negative control (P>0.05). These data showed that ev21 integration was not linked with late feathering in chickens. PCR for 7 590 bp is a method for screening of ev21 in chickens. 5'LTR of ev21 had the high promoter activity.

CLC Number:

S852.659.3

WANG Qi, WANG Han, ZHANG Xiu-ling, LIU Chun-yang, ZHANG Le-chao, LI Lan-hui, LI Xiang-long. Analysis of Non-linkage between Endogenous ALV-ev21 and Late Feathering of Chickens and Its Promoter Activity of LTR[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(5): 930-937.

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Analysis of Non-linkage between Endogenous ALV-ev21 and Late Feathering of Chickens and Its Promoter Activity of LTR

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